Coding

Part:BBa_K639001:Design

Designed by: Adrian Naas   Group: iGEM11_NTNU_Trondheim   (2011-08-26)


relA (ppGpp synthase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1383
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1383
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1728


Design Notes

Contains PstI site at 1363-1368.


Source

The gene was amplified from E coli DH5 alpha chromosomal DNA using colony PCR with the following primers

References